The strength of this study, and continuous reference intervals in general, is that we did not need to make any assumptions or arbitrary age partitions. Non-parametric continuous pediatric reference intervals for T, [www.foreignspouse.com](https://www.foreignspouse.com/@denisha16n9782) SHBG, BAT and [git.4lcap.com](https://git.4lcap.com/reginaginder51) FT in males (left) and females (right) showing confidence intervals calculated using the sandwich formula . Our report suggests that manufacturers can fill an important gap by providing users with an up-to-date technical data sheet on skinfold calipers. In some Latin American countries, mainly in Brazil, the measurement resolution of the indicator dial and [divitube.com](https://divitube.com/@remonaedelson?page=about) the raw material of the rods are used as criteria to classify skinfold calipers as clinical or scientific. Here we use the original iron and transferrin data from healthy children and adolescents to calculate transferrin saturation and establish age- and sex-specific pediatric reference intervals for this parameter. Here we use the original iron and transferrin data from the CALIPER cohort to establish age- and sex-specific pediatric reference intervals for transferrin saturation. The present study is an extension to these previously determined reference intervals, by establishing age- and sex-specific pediatric reference intervals for transferrin saturation using Abbott assays. This study provides age- and sex-specific reference intervals for percent transferrin saturation based on a multi-ethnic Canadian population aged 0- Oral contraceptive use did not have a significant impact on percent transferrin saturation in our pediatric reference population. Another study showed serum iron, TIBC, and serum transferrin levels were significantly greater in users of oral contraceptives, while transferrin saturation was not different from the control group’s level (27). Additionally, similar to our study, others have demonstrated a small increase in transferrin saturation with age (2,19–21). Although transferrin saturation is statistically different between sexes when comparing the mean and standard deviation of transferrin saturation between sexes, further examination is needed to determine if this difference is clinically significant. As transferrin saturation and CRP were shown to be significantly (negatively) correlated, participants with an elevated CRP value (≥ 10 mg/L) were excluded. For partitions containing 40 participants, the robust statistical method of Horn and Pesce (18) was used to calculate the reference interval. Reference intervals for partitions with a sample size ≥ 120 participants were calculated using the nonparametric rank method. Subjects were chosen to ensure the ethnic composition of study participants was in accordance with the 2006 Canadian census data for the province of Ontario (15). Only a few studies have investigated total T reference intervals using LC-MS/MS in pediatric populations , , , , . Point estimates of the reference intervals are selected at the mid-point of each respective age-bin. Here we provide a workflow for non-parametric continuous reference intervals applied to T, FT, BAT, and SHBG using the R language quantregGrowth package. Non-parametric continuous reference intervals methods have previously been developed to avoid some of these drawbacks. These facts do not invalidate the use of any other type of original or generic skinfold caliper mentioned or not in this study, such as the Holtain® (Holtain Ltd, UK). Therefore, it is absolutely necessary to regulate an international protocol of construction standards for skinfold calipers. The predictor variable is age, but in this case it’s embedded in another function, ps(), which is a spline. In our case, the dependent variable is T (the column of data arbitrarily titled t_nmol_l in the dataframe). The gcrq function, an acronym for growth chart regression quantile, is part of the quantregGrowth package and has a series of required of arguments ("parameters"). Breaking down the last lines of the cross-validation call shown above, we used the function for the continuous age-dependent centile curve, gcrq. The lower 2.5th and [angdesh.com](https://angdesh.com/author/sheltoncomb/) upper 97.5th centiles were modeled using the quantregGrowth, , package using R version 4.1. For the 4-site body fat calipers test, men and women take the same measurements (in millimeters) but use different equations to calculate their body fat percentage depending on their age. For the 9-site body fat calipers test, men and women take the same measurements and use the same equations to calculate their body fat percentage. For the 7-site body fat calipers test, men and women take the same measurements but use different equations to calculate their body fat percentage. For example, if you’re a man around 8 or 9% body fat, skinfold calipers will likely give you a reading closer to 5%. In leaner people, however (less than 15% for men or 25% for women), body fat calipers tend to underestimate body fat percentage, with the results becoming more skewed the leaner you get. For people with average or above-average amounts of body fat (15% and higher for men, or 25% and higher for women), body fat calipers are a fairly accurate way to measure body fat percentage. Our transferrin saturation reference intervals demonstrated a slight sex difference, with males having higher transferrin saturation than females after 14 years of age. In this study, we establish pediatric reference intervals for transferrin saturation using an Abbott platform. Age- and sex-specific pediatric reference intervals for percent transferrin saturation are shown in Table 1. In our first publication of 40 routine biochemical markers we established age- and sex-specific reference intervals for serum iron and transferrin (4). While these do not seem incompatible with the present study, detailed comparison is not possible. In this context, [https://hero-cloud-stg-code.cnbita.com/stantonvanraal](https://hero-cloud-stg-code.cnbita.com/stantonvanraal) a dedicated (i.e. binned) analysis of results for patients less than 1 year of age may be warranted. 3 showing reasonable agreement among the studies bearing in mind that the statistical analyses are performed differently and samples are differentially binned into age-categories. SHBG showed varying patterns with age, differing between males and [123.57.225.51](http://123.57.225.51:3000/cherie77a55417) females. In both males and females, T values peaked in the age range of puberty, males being ≃10× higher.